<p class="jove_content">This protocol describes a method using esophageal squamous cell carcinoma cell lines as an example to analyze protein lactylation. The process involves analyzing protein lactylation from cell culture to confirmation of candidates, including lactylation-based mass spectrometry analysis and confirmation by immunoprecipitation.</p>

mass-spectrometry-based-lactylome-confirmation-lactylated

Mass Spectrometry-based Lactylome and Confirmation of Lactylated Proteins

First identified in 2019, lactylation, a new post-translational modification (PTM) on lysine residues, has since been shown to be of interest in multiple ...

Institution - Centre de Recherche du Centre Hospitalier de l'Université de Sherbrooke (CRCHUS)

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