Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.

studying-interactions-between-myeloid-cells-car-t-cells-vitro

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

Chimeric antigen receptor T (CART) cell therapy has led to remarkable clinical successes treating hematological malignancies. However, most patients ...

A method to generate a doxorubicin-induced cardiomyopathy model in adult zebrafish (Danio rerio) is described here. Two alternative ways of intraperitoneal injection are presented and conditions to reduce variations among different experimental groups are discussed.

a-doxorubicin-induced-cardiomyopathy-model-in-adult-zebrafish

A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish

The genetically accessible adult zebrafish (Danio rerio) has been increasingly used as a vertebrate model for understanding human diseases such as ...

A rapid way to conduct immunostaining of zebrafish embryonic heart is described. Compared to the whole mount immunostaining approach, this method dramatically increases the penetration of the antibodies, which allows obtaining high resolution images that reveal cellular/subcellular structures in the heart within a much reduced processing time.

immunostaining-of-dissected-zebrafish-embryonic-heart

Immunostaining of Dissected Zebrafish Embryonic Heart 

Zebrafish embryo becomes a popular in vivo vertebrate model for studying cardiac development and human heart diseases due to its advantageous embryology ...

Institution - Mayo Clinic Graduate School of Biomedical Sciences

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