Aseptic Laboratory Techniques: Plating Methods

63.2K 조회수

490회 인용됨

18:00 분

2012년 5월 11일

10.3791/3064-v

2012년 5월 11일

63.2K 조회수

When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.

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Aseptic Technique

Chapters in this video

0:05

Title

1:51

A Safe and Sterile Workplace

3:04

Streak Plate Procedure: Isolation of Bacterial Colonies using the Quadrant Method

5:26

Pour Plate Procedure: Enumeration of Bacterial Cells in a Mixed Sample

6:38

Spread-plating: Turntable with Glass or Metal Rod

7:59

Spread-plating: the Copacabana Method

9:11

Soft Agar Overlay Procedure: Plaque Assay

11:52

Replica Plate Protocol: Screening Mutants and Auxotrophs

13:59

Clean Up

14:25

Practical Applications

17:22

Conclusion

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