Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

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07:51 мин.

20 марта 2026 г.

10.3791/69853-v

20 марта 2026 г.

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Neal T. Ramseier¹ , Herath D.W. Herath¹ , Alyssa Burgess¹ , Hirushi Gunasekara¹ , Ying S. Hu¹^,²^,³^,⁴

¹Department of Chemistry, University of Illinois Chicago, ²University of Illinois Cancer Center, ³Department of Biomedical Engineering, University of Illinois Chicago, ⁴Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois Chicago

This protocol describes single-antibody labeling (SAL) to resolve the nanoscale spatial organization of plasma membrane proteins. By leveraging cumulative antibody-epitope interactions at the single-molecule level, membrane SAL (mSAL) maps local epitope distributions while simultaneously capturing antibody binding behavior in the native cellular environment.